Transformative influence of Afro-Brazilian syncopation on European compound melodies in Brazilian Choro music melorhythmical re-organization of interleaved melodic structures in progressive Afro-Brazilian music from the late-19th century

During the second half of the 19th century, Rio de Janeiro’s public life became the setting for spontaneous gatherings by a varied assortment of amateur musicians. After attracting professional musicians from Rio’s popular entertainment circuit, these meetings evolved into creative musical hubs during which songs of the day were instrumentally re-styled. Commonly known as ‘Choro’ or ‘Chorinho’ (Port. ‘cry’), these music practices were infused with melodic elements from classical chamber music as well as stylistic influence from popular dance music. One significant development was the introduction of compound melodies, single-note structures that elicit the impression of being multi-melodic. Influenced by Afro-Brazilian accompanists, compound melodies became infused with syncopated phrasing elements and evolved into one of Choro’s most emblematic traits. This thesis focuses on factors and processes that created Choro’s syncopated compound melodies, identifying and explaining their organizational transformation during Choro’s stylistic hybridization processes

Plant-based production of a protective vaccine antigen against the bovine parasitic nematode Ostertagia ostertagi

Abstract The development of effective recombinant vaccines against parasitic nematodes has been challenging and so far mostly unsuccessful. This has also been the case for Ostertagia ostertagi, an economically important abomasal nematode in cattle, applying recombinant versions of the protective native activation-associated secreted proteins (ASP). To gain insight in key elements required to trigger a protective immune response, the protein structure and N-glycosylation of the native ASP and a non-protective Pichia pastoris recombinant ASP were compared. Both antigens had a highly comparable protein structure, but different N-glycan composition. After mimicking the native ASP N-glycosylation via the expression in Nicotiana benthamiana plants, immunisation of calves with these plant-produced recombinants resulted in a significant reduction of 39% in parasite egg output, comparable to the protective efficacy of the native antigen. This study provides a valuable workflow for the development of recombinant vaccines against other parasitic nematodes